CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
نویسندگان
چکیده
The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.
منابع مشابه
Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans.
We have achieved targeted heritable genome modification in Caenorhabditis elegans by injecting mRNA of the nuclease Cas9 and Cas9 guide RNAs. This system rapidly creates precise genomic changes, including knockouts and transgene-instructed gene conversion.
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